The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
IN VITRO EVALUATION OF CEPHACETRILE, A NEW CEPHALOSPORIN ANTIBIOTIC
HAROLD C. NEUELAINE B. WINSHELL
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JOURNAL FREE ACCESS

1972 Volume 25 Issue 7 Pages 400-404

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Abstract

Cephacetrile, 7-cyanacetamido-cephalosporanic acid, is a new cephalosporin active against many gram-positive and gram-negative organisms. Staphylococcus aureus and Streptococcus pyogenes were inhibited by less than 1 μg/ml, but 25 μg/ml were needed to inhibit enterococci. The majority of Escherichia coli and Klebsiella strains and Proteus mirabilis strains were inhibited by 12.5μg/ml. Serratia, indole-positive Proteus, Pseudomonas and Enterobacter strains were resistant to more than 100 μg/ml. The in vitro activity of cephacetrile was influenced by inoculum size. Tube dilution and agar-plate dilution methods used to determine MIC differed by two to eight-fold. Cephacetrile was hydrolyzed by β-lactamases of intact E. coli, S. typhymurium, E. cloacae, P. morganit, Ps. aeruginosa strains. Hydrolysis of cephacetrile by purified β-lactamases showed that cephacetrile was not hydrolyzed as rapidly as cephalothin or cephaloridine.

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© Japan Antibiotics Research Association
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