THE INDUCTION OF AN ATP-ENERGIZED MITOCHONDRIAL VOLUME CHANGE BY THE COMBINATION OF THE -S-S- COMPOUND, ELLMAN'S REAGENT WITH EITHER A RESPIRATORY INHIBITOR OR AN UNCOUPLING AGENT

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When the -S-S- compound ELLMAN'S reagent was combined either with the respiratory inhibitor antimycin or the uncoupling agent 2, 4-dinitrophenol an ATP-energized mitochondrial volume change was induced. Thus ELLMAN'S reagent behaved as an electrophilic thiol reagent analogous to showdomycin and N-ethylmaleimide. These results encouraged the search for possible reactions between the pivotal mitochondrial thiol group and biologically important substances which contain the -S-S- grouping even though older reports in the literature of the induction of mitochondrial swelling by biologically important -S-S- compounds were recently discounted.