1973 Volume 26 Issue 12 Pages 732-736
Micromonospora sp. 69-683 produces neomycin B and except for an early report of production of actinomycin from a culture not well defined, is presently the only other known example of a major Streptomyces-produced antibiotic also produced by a member of the genus Micromonospora. A variety of carbon and nitrogen sources were found to be suitable substrates for Micromonospora sp. 69-683. The antibiotic is removed from the fermentation broth with a cationic-exchange resin and is purified by passing the mixture through a Dowex 1×2 resin column. Chemical, physical and biological data confirm that it is identical to neomycin B.
