The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
CHARACTERIZATION OF BLEOMYCIN ACTION ON DNA
HIDEKI ASAKURAMAKOTO HORIHAMAO UMEZAWA
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1975 Volume 28 Issue 7 Pages 537-542

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Abstract

Cleavage of SV40 DNA by bleomycin was assayed quantitatively in vitro in the presence of various polynucleotides. SV40 DNA was protected from bleomycin-induced cleavage by native or denatured DNA of other origins, poly dG-C•poly dG-C, poly dA-T•poly dA-T and poly dA-T (denatured) but not by tRNA of E. coli, apurinic acid, poly dA, poly dT and various deoxyribooligonucleotides. Various bleomycins and their derivatives and various fragments of bleomycin were tested for possible activity in cleaving SV40 DNA and from the results some structure-activity relationships for the action of bleomycin to act on DNA were outlined. Actinomycin D stimulated bleomycin action while ethidium bromide inhibited it.

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