HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF NATURAL PRODUCTS

I. SEPARATION OF CEPHALOSPORIN C DERIVATIVES AND CEPHALOSPORIN ANTIBIOTICS; ISOLATION OF CEPHALOSPORIN C FROM FERMENTATION BROTH

Abstract

Microbonded propylamine silica with a solvent system containing acetic acid, methanol, acetonitrile and water (2:4:7.5:86.5) is suitable for an efficient separation of mixtures containing several closely related cephem derivatives. The same system with preparative columns was used for the isolation of cephalosporin C directly from the filtered broth of C. acremonium fermentation.