The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
MICROBIAL REDUCTION OF THE SIDE-CHAIN CARBONYL OF DAUNORUBICIN AND N-ACETYLDAUNORUBICIN
A. A. ASZALOSN. R. BACHURB. K. HAMILTONA. F. LANGLYKKEP. P. ROLLERM. Y. SHEIKHM. S. SUTPHINM. C. THOMASD. A. WAREHEIML. H. WRIGHT
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1977 Volume 30 Issue 1 Pages 50-58

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Abstract

Microorganisms reduced the side-chain carbonyl of daunorubicin to yield 13-dihydrodaunorubicin (daunorubicinol; daunomycinol). This microbial transformation occurred under aerobic conditions in agitated baffled shake flasks incubated at 37°C. The microorganisms were first grown in a medium which supported dense growth. Daunorubicin-HCl was then added. Following a period of incubation, broths were adjusted to pH 10.0 and extracted with chloroform. Daunorubicinol was recovered and purified from the chloroform extracts by preparative TLC. Identity of the daunorubicinol was established by TLC and spectroscopy (UV-vis, IR, NMR, MS, CD and ORD). N-Acetyldaunorubicin was likewise reduced microbially to N-acetyldaunorubicinol. N-Acetyldaunorubicinol appears to be a new compound which is yet to be tested for antitumor activity.

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© Japan Antibiotics Research Association
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