The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
SP127, A BACTERIAL PROTEIN ENHANCING THE ACTIVITY OF MACROLIDE ANTIBIOTICS AGAINST PSEUDOMONAS AERUGINOSA: ISOLATION AND CHARACTERIZATION
MASAKAZU KIKUCHIYOSHIO NAKAO
Author information
JOURNAL FREE ACCESS

1977 Volume 30 Issue 3 Pages 209-214

Details
Abstract

Some strains belonging to the genera Bacillus and Pseudomonas were found to produce in the culture broth, a protein which synergistically enhanced the activity of macrolide antibiotics against Pseudomonas aeruginosa. The protein produced by Bacillus brevis ATCC 8185, designated SP127, was isolated in a pure state by means of ammonium sulfate fractionation, CM-cellulose column chromatography and gel filtration on Sephadex G-100. The sedimentation coefficient and the molecular weight of SP127 were 1.8 s and 15, 000, respectively according to the analytical ultracentrifuge. The isoelectric point of SP127, obtained by electrofocusing experiments, was over 10.0. Half cystine and histidine were absent in the molecule.

Content from these authors
© Japan Antibiotics Research Association
Previous article Next article
feedback
Top