MICROBIAL PRODUCTION OF VITAMIN B₁₂ ANTIMETABOLITES. III

COMPOUND 102804 FROM BACILLUS CEREUS

Abstract

A basic compound with empirical formula C₁₂H₁₆N₂O₅ was isolated from Bacillus cereus
102804 fermentations of a soybean meal - glucose medium. The inhibitory activity of compound 102804 on growth of Gram-positive and Gram-negative bacteria growing in a chemically defined medium was reversed by vitamin B₁₂, by L-methionine, and by D-methionine. It has no inhibitory activity for Escherichia coli (Davis 113-3) when grown in media containing L-methionine.
The biosynthesis of 102804 coincides with the sporulation of the B. cereus, and the compound is not produced in the absence of sporulation.