Ro 13-9904: AFFINITY FOR PENICILLIN BINDING PROTEINS AND EFFECT ON CELL WALL SYNTHESIS

Abstract

Studies on the binding of Ro 13-9904, a new broad-spectrum cephalosporin, showed that it had higher affinities for PBPs 1b, 2, and 3 of Escherichia coli (3>1b>2) than cefazolin, cephaloridine, cephalothin or cephalexin. With Haemophilus influenzae, Ro 13-9904 showed highest affinities for PBPS 4 and 5 followed by PBP 2. It inhibited total cell wall synthesis at lower concentrations than the other β-lactam antibiotics tested.