The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
A METHOD FOR SEPARATING COMMERCIAL COLISTIN COMPLEX INTO NEW COMPONENTS: COLISTINS PRO-A, PRO-B AND PRO-C
YUKIO KIMURAHISAMI KITAMURAKYOZO HAYASHI
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1982 Volume 35 Issue 11 Pages 1513-1520

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Abstract

Commercial colistin was separated into three major components as well as lipid components by reversed phase adsorption chromatography on Diaion HP-20 AG, a macroreticular styrene-divinylbenzene copolymer, without any inorganic salts or detergents, in aqueousorganic solvent as mobile phase. These expected components were colistins A, B and C; there were, however, appreciable differences between these components and colistins A, B and C, isolated by countercurrent distribution. The newly isolated components showed slightly higher potency than colistins A, B and C; their molecular weights, as determined by gel permeation chromatography of 2, 4-dinitrophenyl derivatives on TSK-GEL G2000H (mobile phase: dimethylformamide), were also slightly higher. Accordingly, they were tentatively named colistins pro-A, pro-B and pro-C. During purification by countercurrent distribution (solvent system: sec-BuOH-n-BuOH-0.1 N HCl, 30: 6: 40), colistin pro-A was converted to colistin A. Similarly, colistin pro-B was converted to colistin B, and colistin pro-C to colistin C. Therefore, we concluded that colistins A, B and C are artifacts.

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