HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) OF NATURAL PRODUCTS V

THE USE OF HPLC IN THE CELL-FREE BIOSYNTHETIC CONVERSION OF α-AMINOADIPYL-CYSTEINYL-VALINE (LLD) INTO ISOPENICILLIN N

Abstract

Isopenicillin N (I) and penicillin N (II) were separated effectively by reversed phase HPLC as derivatives of chiral 2, 3, 4, 6-tetra- O-acetyl-β-D-glucopyranosyl isothiocyanate (III). This permitted identification of isopenicillin N isolated by HPLC during a biosynthetic cell-free experiment using α-L-aminoadipyl-L-cysteinyl-D-valine (IV) as precursor.