ACLACINOMYCIN A-INHIBITION OF PHAGE φX174 DNA SYNTHESIS HIN VITRO

Abstract

Aclacinomycin A inhibited the in vitro conversion of phage φX174 single-stranded DNA to the replicative form DNA. DNA synthesis was inhibited by 50% in the presence of 15μM aclacinomycin A. The inhibition was competitive with respect to template DNA (K_i=13 μM) and was reversed by addition of Escherichia coli cell extracts. Short complementary strands approximately one-third of unit length molecule were synthesized in the presence of 15 μM aclacinomycin A. The data suggest that aclacinomycin A may inhibit the process of φX174 DNA chain elongation by a direct interaction with the E. coli host enzymes.