INTERACTION OF ANTHRACYCLINE ANTIBIOTICS WITH BIOPOLYMERS

VIII. BINDING PARAMETERS OF ACLACINOMYCIN A TO DNA

Abstract

The binding of aclacinomycin A to DNA was investigated spectrophotometrically under equilibrium conditions. The self-association behaviour of aclacinomycin A was identified as dimerization. Based on a model of overlapping potential binding sites the subsequent results were obtained: equilibrium constant of cooperative binding K=(7.58±2.15)×10⁶ M^-1, size of a binding site α=3.98± 0.14 base pairs, cooperativity parameter σ=0.12±0.10. These parameters were compared with those of adriamycin, daunomycin, and iremycin to draw some conclusions regarding the structural specialities of aclacinomycin A.