STUDIES ON WF-5239, A NEW POTENT PLATELET AGGREGATION INHIBITOR

Abstract

WF-5239 was produced by a fungal strain identified as Aspergillus fumigatus Fresenius. The substance was purified by solvent extraction followed by chromatography on silica gel and then crystallized (C₉H₉NO₂, mp 142-145°C). The chemical structure was determined from its physico-chemical properties as N-[2-cis(4-hydroxyphenyl)ethenyl]formamide. WF-5239 has inhibitory activity against rabbit platelet aggregation induced by arachidonic acid and collagen, with IC₅₀ values of 1.25 and 5.0μg/ml, respectively. Arachidonic acid induced mortality in mice was reduced by a single intraperitoneal dose of WF-5239 (30 mg/kg).These biological properties have been compared with those of aspirin.