A NEW MACROMOLECULAR ANTITUMOR ANTIBIOTIC, C-1027
II. ISOLATION AND PHYSICO-CHEMICAL PROPERTIES
1988 年 41 巻 11 号 p. 1580-1585
詳細
1988 年 41 巻 11 号 p. 1580-1585
A new macromolecular antibiotic C-1027 was obtained from the broth filtrate of Streptomyces ghbisporus C-1027 by precipitation with ammonium sulfate, DEAE-cellulose column chromatography and gel filtration chromatography on a Sephadex G-75 column. This antibiotic, prepared as a white powder, is an acidic polypeptide having an isoelectric point of pH 3.5-3.7 and a molecular weight of 15, 000 as determined by SDS-polyacrylamide gel electrophoresis and gel filtration chromatography. The acid hydrolysate of the purified antibiotic C-1027 contained no methionine or tryptophan. From the physico-chemical data, it may be considered to possess a very labile non-protein chromophore.
