1988 Volume 41 Issue 12 Pages 1854-1861
The effect of a potent antitumor antibiotic, kazusamycin B, on the cell cycle of LI210 cells was examined. Kazusamycin B arrested synchronized LI210 cells at Gl phase. Retardation of metaphase initiation was also observed. Flow cytometric analysis of kazusamycin B-treated asynchronized cells also confirmed Gl arresting effect of kazusamycin B. In addition, an unidentified cell population with lower fluorescence intensity than Gl population was observed when the cells were exposed to the drug longer than 12 hours. Morphology of kazusamycin B-treated LI210 cells revealed that the intranuclear structure changed within 4 hours, and that abnormal condensation of nuclei coincided with the appearance of unidentified population. Kazusamycin B inhibited RNA synthesis moderately but specifically at 2 hours. However, this inhibition might be a secondary effect of the antibiotic-induced structural abnormality of the nuclei.
