The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
COMPLESTATIN, A POTENT ANTI-COMPLEMENT SUBSTANCE PRODUCED BY STREPTOMYCES LAVENDULAE
I. FERMENTATION, ISOLATION AND BIOLOGICAL CHARACTERIZATION
ISAO KANEKOKATUO KAMOSHIDASHUJI TAKAHASHI
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1989 Volume 42 Issue 2 Pages 236-241

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Abstract

A new potent inhibitor of complement system, named complestatin, was isolated from the mycelium of Streptomyces lavendulae SANK 60477. Complestatin (C61H45N7O15Cl6, MW 1, 325) was a peptide compound having two unusual amino acids, D-(-)-4-hydroxyphenylglycine and D-(-)-3, 5-dichloro-4-hydroxyphenylglycine. This compound inhibited the hemolysis of sensitized sheep erythrocytes (EA) mediated by guinea pig and human complement 50% at concentrations of 0.4 and 0.7 μg/ml, respectively, but did not trypsin and α-chymotrypsin activities at 200 μg/ml. When complestatin was administered intravenously to the sensitized guinea pigs, it strongly inhibited the systemic anaphylactic shock elicited by the antigen probably by blocking generation of anaphylatoxins (C3a and C5a).

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