PRODUCTION, ISOLATION AND STRUCTURE DETERMINATION OF A NOVEL β-GLUCOSIDASE INHIBITOR, CYCLOPHELLITOL, FROM PHELLINUS SP.

Abstract

In the course of our screening of β-glucosidase inhibitor, a culture filtrate of a mushroom, Phellinus sp. strongly inhibited the enzyme activity. The active substance was isolated through charcoal separation, column chromatography and crystallization. Spectroscopic and crystallographic analysis revealed that it had a novel cyclitol structure, (1S, 2R, 3S, 4R, 5R, 6R)-5-hydroxymeihy¥-loxabicyclo[4, l, 0]heptane-2, 3, 4-triol, and we named it cyclophellitol. It inhibited almond-derived β-glucosidase with an IC₅₀ of 0.8μg/ml.