TOPOSTIN, A NOVEL INHIBITOR OF MAMMALIAN DNA TOPOISOMERASE I FROM FLEXIBACTER TOPOSTINUS SP. NOV. I. TAXONOMY, AND FERMENTATION OF PRODUCING STRAIN

KENICHI SUZUKI; HIROSHI YAMAGUCHI; SIGERU MIYAZAKI; KOUJI NAGAI; SHUN-ICHI WATANABE; TAKESHI SAITO; KAZUYUKI ISHII; MINORU HANADA; TAKESHI SEKINE; YOJI IKEGAMI; TOSHIWO ANDOH

doi:10.7164/antibiotics.43.154

TOPOSTIN, A NOVEL INHIBITOR OF MAMMALIAN DNA TOPOISOMERASE I FROM FLEXIBACTER TOPOSTINUS SP. NOV.

I. TAXONOMY, AND FERMENTATION OF PRODUCING STRAIN

KENICHI SUZUKI, HIROSHI YAMAGUCHI, SIGERU MIYAZAKI, KOUJI NAGAI, SHUN-ICHI WATANABE, TAKESHI SAITO, KAZUYUKI ISHII, MINORU HANADA, TAKESHI SEKINE, YOJI IKEGAMI, TOSHIWO ANDOH

Author information

KENICHI SUZUKI
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
HIROSHI YAMAGUCHI
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
SIGERU MIYAZAKI
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
KOUJI NAGAI
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
SHUN-ICHI WATANABE
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
TAKESHI SAITO
Bioscience Research Laboratories, Central Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd.
KAZUYUKI ISHII
Department of Hygienic Chemistry, Meiji College of Pharmacy
MINORU HANADA
Department of Hygienic Chemistry, Meiji College of Pharmacy
TAKESHI SEKINE
Department of Hygienic Chemistry, Meiji College of Pharmacy
YOJI IKEGAMI
Department of Hygienic Chemistry, Meiji College of Pharmacy
TOSHIWO ANDOH
Department of Hygienic Chemistry, Meiji College of Pharmacy
Present address: Aichi Cancer Center Research Institute, Laboratory of Biochemistry

JOURNAL FREE ACCESS

1990 Volume 43 Issue 2 Pages 154-157

DOI https://doi.org/10.7164/antibiotics.43.154

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Abstract

We found a new inhibitor of mammalian DNA topoisomerase I, named topostin, from a bacterial culture. The bacteria was identified as Flexibacter topostinus sp. nov., B-572. Morphological and physiological characteristics, and utilization of sugars were examined. Comparison of the strain with known species of the genus Flexibacter was made and indicates that the strain is a new species of the genus Flexibacter. The bacteria produced the inhibitor in parallel with their growth up to 72 hours.

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