A NEW INHIBITOR OF PROTEIN KINASE C, RK-286C (4'-DEMETHYLAMINO-4'-HYDROXYSTAUROSPORINE)

II. ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND STRUCTURE

Abstract

RK-286C, a new inhibitor of protein kinase C, has been found by the bleb-forming assay using K562 cells. It was produced by Streptomyces sp. RK-286 and purified by solvent extraction, silica gel chromatography and preparative HPLC. Spectrometric analysis revealed that the structure is 4'-demethylamino-4'-hydroxystaurosporine.