The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
MECHANISM OF ACTION OF ATPENIN B ON RAJI CELLS
KAZUSHI OSHINOHIDETOSHI KUMAGAIHIROSHI TOMODASATOSHI OMURA
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1990 Volume 43 Issue 9 Pages 1064-1068

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Abstract

Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM).The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13-0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5'-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting the inhibition of ATP-generating system by atpenin B.

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