The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
ISOLATION AND PARTIAL CHARACTERISATION OF ACV SYNTHETASE FROM Cephalosporium acremonium AND Streptomyces clavuligerus
EVIDENCE FOR THE PRESENCE OF PHOSPHOPANTOTHENATE IN ACV SYNTHETASE
JACK E. BALDWINJULIETTE W. BIRDROBERT A. FIELDNIAMH M. O'CALLAGHANCHRISTOPHER J. SCHOFIELDANTHONY C. WILLIS
Author information
JOURNALS FREE ACCESS

1991 Volume 44 Issue 2 Pages 241-248

Details
Abstract

δ-(L-α-Aminoadipoyl)-L-cysteinyl-D-valine (ACV) synthetase was isolated and partially characterised from Cephalosporium acremonium CO728 and Streptomyces clavuligerus. The purification procedure resulted in a 745- and 277-fold increase in specific enzyme activity, respectively. Both enzymes had similar apparent molecular masses of ca. 300 kdaltons by SDS-polyacrylamide electrophoresis, under reducing and denaturing conditions, and in excess of 600 kdaltons in the native state by gel filtration. Attempts to obtain an N-terminal amino acid sequence of ACV synthetase from C. acremonium were unsuccessful, hence internal amino acid sequence data were obtained after tryptic digestion of the protein. Phosphopantothenic acid was shown to be associated with the enzyme from both sources, which suggests the possible involvement of pantothenate as a 'swinging arm' in the formation of the tripeptide ACV.

Information related to the author
© Japan Antibiotics Research Association
Previous article Next article
feedback
Top