LEUHISTIN, A NEW INHIBITOR OF AMINOPEPTIDASE M, PRODUCED BY Bacillus laterosporus BMI156-14F1 I. TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES

TAKAAKI AOYAGI; SHIGEMI YOSHIDA; NAOKO MATSUDA; TAKAKO IKEDA; MASA HAMADA; TOMIO TAKEUCHI

doi:10.7164/antibiotics.44.573

LEUHISTIN, A NEW INHIBITOR OF AMINOPEPTIDASE M, PRODUCED BY Bacillus laterosporus BMI156-14F1

I. TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES

TAKAAKI AOYAGI, SHIGEMI YOSHIDA, NAOKO MATSUDA, TAKAKO IKEDA, MASA HAMADA, TOMIO TAKEUCHI

著者情報

ジャーナルフリー

1991 年 44 巻 6 号 p. 573-578

DOI https://doi.org/10.7164/antibiotics.44.573

詳細

抄録

Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 × 10^-7 M.

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