APHE-1 AND APHE-2, TWO NEW ANTIMICROBIAL AND CYTOTOXIC ANTIBIOTICS

II. PHYSICO-CHEMICAL PROPERTIES AND STRUCTURE ELUCIDATION

Abstract

Two new pyrazolo-isoquinolinone antibiotics, APHE-1 and APHE-2, have been isolated from the culture nitrate and mycelia of Streptoverticillium griseocarneum NCIMB 40447. Molecular formulae were established as C₁₃H₁₂N₂O for APHE-1 and C₁₄H₁₄N₂O for APHE-2, by elemental analysis, NMR and mass spectra. 2D NMR techniques (¹H-¹H COSY-45 and ¹H-¹³C correlated spectroscopy) have been applied to establish their structures.