CONFIRMATION OF IN VITRO SYNTHESIS OF 2-DEOXY-SCYLLO-INOSOSE, THE EARLIEST INTERMEDIATE IN THE BIOSYNTHESIS OF 2-DEOXYSTREPTAMINE, USING CELL FREE PREPARATIONS OF STREPTOMYCES FRADIAE

Abstract

The in vitro activity of formation of 2-deoxy-scyllo-inosose, the earliest intermediate of the 2-deoxystreptamine biosynthesis, was confirmed in the precipitate fraction obtained by 30-45% saturation of ammonium sulfate from the 15, 000×g supernatant of the sonicated Streptomyces fradiae IFO 13147 cells.