2001 Volume 54 Issue 2 Pages 157-165
Studies on the susceptibility of pathogenic Nocardia to macrolide antibiotics, chalcomycin and tylosin, showed that most of the Nocardia species examined were highly resistant to both antibiotics, although N. nova was moderately susceptible. N. asteroides IFM 0339 converted these macrolides into inactive metabolites by glycosylation at 2'-OH or glycosylation and reduction of the 20-formyl group. The structures of the metabolites were determined from NMR and MS data to be 2'-[O-(β-D-glucopyranosyl)]chalcomycin (2), 2'-[O-(β-D-glucopyranosyl)]tylosin (5) and 20-dihydro-2'-[O-(β-D-glucopyranosyl)]tylosin (4).