New Cell-cell Adhesion Inhibitors from Streptomyces sp. UMA-044

Abstract

Three compounds, NP25301 (1), NP25302 (2) and bohemamine (3), inhibitors of cell adhesion based on LFA-1/ICAM-1, were isolated from the cultured broth of the strain Streptomyces sp. UMA-044. New compounds 1 and 2 were identified as 2-(3'-carbamoylphenoxy) acrylic acid methyl ester and deoxybohemamine, respectively, based on spectroscopic analyses. Compounds 1-3 inhibited adhesion of HL-60 cells to CHO-ICAM-1 cells at IC₅₀ values of 29.5μg/ml for 1, 24.3μg/ml for 2, and 27.2μg/ml for 3.