Archives of Histology and Cytology
Online ISSN : 1349-1717
Print ISSN : 0914-9465
ISSN-L : 0914-9465
Review articles
Acid-sensing ion channels in taste buds
Shoichi ShimadaTakashi UedaYusuke IshidaTakashi YamamotoShinya Ugawa
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2006 Volume 69 Issue 4 Pages 227-231

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Abstract

Taste receptor cells detect gustatory stimuli using a complex arrangement of ion channels, G protein-coupled receptors, and signaling cascades. Sour and salty tastes are detected by ion channels in the rat. Using a combination of homology screening and functional expression approaches, we screened a rat circumvallate papilla cDNA library and identified acid-sensing ion channel-2a (ASIC2a) and ASIC2b as candidates for the rat sour-sensing channels. In situ hybridization and reverse transcription-polymerase chain reaction experiments revealed that ASIC2a and ASIC2b transcripts were localized in taste bud cells. Immunohistochemistry and immunoprecipitation also revealed that both subunits were expressed in a subset of taste cells and that some of the cells expressed ASIC2a/ASIC2b heteromeric assemblies. Electrophysiological studies demonstrated that stimulation of acetic acid produced larger ASIC2 currents than did hydrochloric acid at the same pH. ASIC2a/ ASIC2b channels generated maximal inward currents at pH ≤ 2.0, which agrees well with the in vivo pH-sensitivity of rat taste cells. The amiloride-sensitivity of ASIC2a/ ASIC2b heteromer lessened with decreasing pH and almost completely disappeared at pH 2.0. These data suggest that ASIC2a and ASIC2b may play roles in sour taste transduction.

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© 2006 by International Society of Histology and Cytology
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