Immunohistochemical Evidence Against the Coexistence of a Corticotropin-Releasing Factor and Oxytocin or Vasopressin in the Rat Paraventricular Nucleus

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A corticotropin-releasing factor (CRF), Oxytocin and arginine-vasopressin were localized immunohistochemically in the paraventricular nucleus of colchicine-treated male rats. Small CRF-containing neurons were distributed in the parvocellular region. Some magnocellular neurons were also immuno-stained with anti-CRF serum, but after preabsorption of the antiserum with pure synthetic CRF, the staining of these magnocellular neurons was unaffected, which suggests that this CRF-positive reaction was non-specific. By comparing the distribution of CRF and oxytocin or vasopressin on serial 5μm cryostat sections, it was revealed that CRF and oxytocin or CRF and vasopressin do not coexist in the same neuron.