2006 Volume 57 Issue 2 Pages 155-164
Internal transcribed spacer (ITS) region of 18-26S nuclear ribosomal DNA (nrDNA) in 18 species and three subspecies of Pinguicula distributed in the northern hemisphere was sequenced. Based on the ITS results, species in Section Pinguicula were basically well-agreed with the orthodox classification. Although three subspecies of P. longifolia have been described, the present approach showed them to be separate species. The ITS sequences between P. vulgaris and P. bohemica were identical and suggested that the two species could be conspecific as normally treated. Pinguicula alpina did not show any direct relationship with P. ramosa and P. variegata although they have been taxonomically placed in Section Micranthus. The present ITS study suggested that P. villosa, only the species of Section Nana, was closely related to P. ramosa and P. variegata. The northern Pinguicula taxa studied here performed the same seasonal growth pattern, but it was likely as a result of convergent evolution.