2020 Volume 68 Issue 1 Pages 79-88
“FA100” of eugenol formulation is the only fish anesthetic that obtained the manufacturing approval as veterinary drugs in Japan. Since vaccines are widespread in Japan, we examined anesthetic effect and safety of “FA100” prior to vaccination for juveniles of red sea bream (Pagrus major), yellowtail (Seriola quinqueradiata), amberjack (S. dumerili), striped jack (Pseudocaranx dentex), rock bream (Oplegnathus fasciatus) and spotted knifejaw (O. punctatus). Although the test fish species and fish body weight are different, the concentration of 100 to 200 ppm of “FA100” is considered to be safe for vaccination work time of the test fish for anesthesia. However, more than 100 g in body weight of amberjack died without recovery from anesthesia. This study was recognized as a guide for the optimal anesthesia concentration and time for vaccinations in marine fishes in Japanese fish farms.