2023 Volume 60 Pages 42-49
We tested the inhibitory effects of nine kinds of essential oil and ethanol on the growth of four strains of fungi (i.e. Cladosporium cladosporides NBRC 4459, C. sphaerospermum NBRC 6348, Aureobasidium pullulans NBRC 6353, Aspergillus oryzae ANCT-07058) using direct contact experiment based on potato-dextrose-agar medium dilution method. The essential oils were a bergamot oil, a cedar one, a clove one, a eucalyptus one, a heliotrope one, a lavender one, a lemon one, a peppermint one, and a rose one. The minimal inhibitory concentration (MIC) of the bergamot oil, the cedar one, the eucalyptus one, and the lemon one to NBRC 6348 and ANCT-07058 were over 2% (v/v) at 25°C. MIC of the lavender oil and the peppermint oil were 0.3-0.5% (v/v) to the four strains. MIC of the clove oil, the heliotrope one and the rose one were 0.025-0.1% (v/v) to all strains. MIC of the rose oil and ethanol was 0.05-0.1% (v/v) and over 2% (v/v), respectively. On the other hand, MIC of the rose oil combined with 2% (v/v) ethanol was 0.025% (v/v) to the four strains.