2015 Volume 2 Issue 2 Pages 19-22
We developed a technique to determine the sex of the sika deer (Cervus nippon) using its hair root by the LAMP method. The SRY gene on the Y chromosome and ZFX gene on the X chromosome were used for sex discrimination. If these two genes were amplified and colored, the deer was a male, and if only the ZFX gene was amplified and colored, it was a female. In order to efficiently amplify the target gene from the hair root of the sika deer, we investigated the reaction conditions. The LAMP method was performed at 64℃ for 40 min. We showed that the LAMP method could be used to accurately identify the sex of this Japanese deer, regardless of strain differences.