Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biological Chemistry Notes
A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography
Koichi UEMURA, Ayako SHIMOIDE, Akiho YOKOTA
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Keywords: anion-exchange chromatography, assay, phosphoglycerate, ribulose 1,5-bisphosphate, ribulose 1,5-bisphosphate carboxylase/oxygenase
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Volume 62 (1998) Issue 3 Pages 567-569

DOI https://doi.org/10.1271/bbb.62.567
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Abstract

  A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with 14CO2.

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© 1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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