Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Notes
Aspartate Decarboxylation Encoded on the Plasmid in the Soy Sauce Lactic Acid Bacterium, Tetragenococcus halophila D10
Takeshi HIGUCHIKinji UCHIDAKeietsu ABE
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1998 Volume 62 Issue 8 Pages 1601-1603


  Tetragenococcus halophila D10 decarboxylates aspartate to alanine, but T. halophila D10 derivatives generated by a curing treatment could not (Asd- derivatives). We observed by electrophoresis three plasmid bands in T. halophila D10; all Asd- derivatives lost the largest of these bands. This plasmid, pD1, has two SalI sites. We cloned and sequenced the 10 kb SalI fragment. The DNA sequence suggests that this fragment contains the aspartate decarboxylating trait.

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© 1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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