Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Analytical Chemistry Note
Effects of Methanol and Temperature on Enzyme Immunoassay with Monoclonal Antibodies Specific to the Insecticide Etofenprox
Masanao KATAGIRITomoko KADOYAKennichi MIYAKEFumihide ISHIBASHIHideo OHKAWA
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1999 Volume 63 Issue 11 Pages 1988-1990

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Abstract

  We examined the effects of methanol and temperature on the reactivity of monoclonal antibodies specific to the insecticide etofenprox. When the antigen-antibody reaction was done at 4°C in 10% methanol, the sensitivity in the enzyme immunoassay with each antibody was more than 10-fold higher than that measured at 37°C. Although in 10% methanol one of the antibodies reacted equally with both etofenprox and the carbonate-derivative of etofenprox, in 50% methanol the antibody reacted with etofenprox, but not with the derivative.

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© 1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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