Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Transcription of emrKY is Regulated by the EvgA-EvgS Two-Component System in Escherichia coli K-12
Akinori KATOHiroe OHNISHIKaneyoshi YAMAMOTOEiji FURUTAHiroyuki TANABERyutaro UTSUMI
Author information
JOURNALS FREE ACCESS

2000 Volume 64 Issue 6 Pages 1203-1209

Details
Abstract

  Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgS1 and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top