Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Two Genes Encoding Fruit Body Lectins of Pleurotus cornucopiae: Sequence Similarity with the Lectin of a Nematode-Trapping Fungus
Naoto IIJIMAHiroto YOSHINOLim Chee TENAkikazu ANDOKatsunori WATANABEYoshiho NAGATA
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2002 Volume 66 Issue 10 Pages 2083-2089

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Abstract

  Our previous studies on the fruit body lectin of Pleurotus cornucopiae revealed the existence of three isolectins, composed of two homodimers and one heterodimer of 16- and 15-kDa subunits. In this study, two genes encoding the lectins were cloned and characterized. Both genes encoded 144 amino acids and only 5 amino acids were different within the coding region, but the nucleotide sequences of the 5′-upstream and 3′-downstream regions differed extensively. Southern hybridization with gene-specific probes showed that one gene encoded the 16-kDa and the other encoded the 15-kDa subunit. Functional lectins were synthesized in Escherichia coli under the direction of these genes. On SDS-PAGE, the recombinant lectins showed the same banding patterns as the native lectins. In amino acid sequence, these lectins showed extensive similarity with the lectin from a nematode-trapping ascomycete fungus, Arthrobotrys oligospora, suggesting that the lectins might also function in capturing nematodes.

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© 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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