Substrate Inhibition of L-Cysteine α,β-Elimination Reaction Catalyzed by L-Cystathionine γ-Lyase of Saccharomyces cerevisiae

Abstract

  The α,β-elimination of L-cysteine catalyzed by Saccharomyces cerevisiae L-cystathionine γ-lyase (EC 4.4.1.1) was inhibited by the substrate. The absorption spectrum of the holoenzyme in the presence of L-cysteine showed that the substrate inhibition observed in this reaction was due mainly to removal of the cofactor.