Gene Expression during P_i Deficiency in Pholiota nameko: Accumulation of mRNAs for Two Transporters

Abstract

  The effects of P_i deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)⁺ RNA isolated from mycelia cultured in P_i-depleted (P⁻) media, and 150 clones corresponding to P_i deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)⁺ RNAs from the mycelia cultured in the P_i-supplied (P⁺) and P⁻ media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during P_i deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to P_i and metabolite transporters.