Cloning and Sequencing of the Serine Dehydrogenase Gene from Agrobacterium tumefaciens

Hisae FUJISAWA; Shinji NAGATA; Emran Kabir CHOWDHURY; Miki MATSUMOTO; Haruo MISONO

doi:10.1271/bbb.66.1137

Biochemistry & Molecular Biology Notes

Cloning and Sequencing of the Serine Dehydrogenase Gene from Agrobacterium tumefaciens

Hisae FUJISAWA, Shinji NAGATA, Emran Kabir CHOWDHURY, Miki MATSUMOTO, Haruo MISONO

Author information

Keywords: serine dehydrogenase, Agrobacterium tumefaciens, short-chain alcohol dehydrogenase, nucleotide sequence, amino acid sequence

JOURNAL FREE ACCESS

2002 Volume 66 Issue 5 Pages 1137-1139

DOI https://doi.org/10.1271/bbb.66.1137

Details

Abstract

The structural gene for NADP+-dependent serine dehydrogenase [EC 1.1.1.-] from Agrobacterium tumefaciens ICR 1600 was cloned into Escherichia coli cells and its complete DNA sequence was analyzed. The gene encodes a polypeptide containing 249 amino acid residues. The enzyme had high sequence similarity to short-chain alcohol dehydrogenases from bacteria and unknown proteins of Haemophilus influenzae, Escherichia coli, and Saccharomyces cerevisiae.

Corresponding author

Register with J-STAGE for free!