Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Organic Chemistry Notes
Green Fluorescent Protein-labeled Recombinant Fluobody for Detecting the Picloram Herbicide
In Seon KIMJae Han SHIMYong Tack SUHKerrm Y. F. YAUJ. Christopher HALLJack T. TREVORSHung LEE
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JOURNAL FREE ACCESS

2002 Volume 66 Issue 5 Pages 1148-1151

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Abstract

  A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC50 value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA).

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© 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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