Cloning and Expression of an Endo-1,6-β-<small>D</small>-glucanase Gene (neg1) from Neurospora crassa

Seiji OYAMA; Youhei YAMAGATA; Keietsu ABE; Tasuku NAKAJIMA

doi:10.1271/bbb.66.1378

Biochemistry & Molecular Biology Notes

Cloning and Expression of an Endo-1,6-β-D-glucanase Gene (neg1) from Neurospora crassa

Seiji OYAMA, Youhei YAMAGATA, Keietsu ABE, Tasuku NAKAJIMA

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Keywords: fungal β-1,6-glucanase, Neurospora crassa, β-1,6-glucanase gene, recombinant β-1,6-glucanase, fungal cell wall

JOURNAL FREE ACCESS

2002 Volume 66 Issue 6 Pages 1378-1381

DOI https://doi.org/10.1271/bbb.66.1378

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Abstract

A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.

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