Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine

Hideaki MATSUOKA; Kanenari OISHI; Masaaki WATANABE; Ikuko KOZONE; Mikako SAITO; Shizunobu IGIMI

doi:10.1271/bbb.67.2459

Food & Nutrition Science Notes

Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine

Hideaki MATSUOKA, Kanenari OISHI, Masaaki WATANABE, Ikuko KOZONE, Mikako SAITO, Shizunobu IGIMI

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Keywords: viable cell detection, fluorescent glucose, fluorescent glycine, foodborne pathogenic bacteria

JOURNAL FREE ACCESS

2003 Volume 67 Issue 11 Pages 2459-2462

DOI https://doi.org/10.1271/bbb.67.2459

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Abstract

The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.

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