Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Communications
Production of Lupin Acid Phosphatase in Transgenic Rice for Use as a Phytate-hydrolyzing Enzyme in Animal Feed
Akira HAMADAKen-ichi YAMAGUCHIMichiko HARADASeiya NIKUMARUJun WASAKITakuro SHINANOHirokazu MATSUIMitsuru OSAKIHideo HONDA
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2004 Volume 68 Issue 7 Pages 1611-1616

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Abstract

The acid phosphatase gene from lupin was expressed in transgenic rice plants under the control of the maize ubiquitin promoter or rice chlorophyll a/b binding protein (Cab) promoter. Transgenic rice leaves exhibited up to an 18-fold increase in phytate-hydrolyzing activity. Based on the phytate-hydrolyzing activity at pH 5.5, more than 85% this activity was retained after heat-treatment at 80 °C for 15 min, and the heterologous enzyme in leaf sections and leaf extracts was relatively stable during storage. A distinct increase in released phosphate was observed when the heterologous enzyme was mixed with the feed extract. These results suggest that the heterologous enzyme in rice plants may maintain its desired characteristics as a phytate-hydrolyzing enzyme when added to animal feed.

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© 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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