Up-Regulation of the Claudin-6 Gene in Adipogenesis

Yeon-Hee HONG; Daisuke HISHIKAWA; Hisae MIYAHARA; Yukihiko NISHIMURA; Hiroaki TSUZUKI; Chizu GOTOH; Tomoyo IGA; Yasuki SUZUKI; Sang-Houn SONG; Ki-Choon CHOI; Hong-Gu LEE; Shinichi SASAKI; Sang-Gun ROH

doi:10.1271/bbb.69.2117

Biochemistry & Molecular Biology Regular Papers

Up-Regulation of the Claudin-6 Gene in Adipogenesis

Yeon-Hee HONG, Daisuke HISHIKAWA, Hisae MIYAHARA, Yukihiko NISHIMURA, Hiroaki TSUZUKI, Chizu GOTOH, Tomoyo IGA, Yasuki SUZUKI, Sang-Houn SONG, Ki-Choon CHOI, Hong-Gu LEE, Shinichi SASAKI, Sang-Gun ROH

Author information

Yeon-Hee HONG
Department of Food Production Science, Faculty of Agriculture, Shinshu University
United Graduate School of Agricultural Science, Gifu University
Daisuke HISHIKAWA
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Hisae MIYAHARA
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Yukihiko NISHIMURA
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Hiroaki TSUZUKI
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Chizu GOTOH
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Tomoyo IGA
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Yasuki SUZUKI
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Sang-Houn SONG
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Ki-Choon CHOI
Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Korea University
Hong-Gu LEE
School of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University
Shinichi SASAKI
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Sang-Gun ROH
Department of Food Production Science, Faculty of Agriculture, Shinshu University

Keywords: claudin-6, adipogenesis, adipose tissue, high-fat diet, fat depot

JOURNAL FREE ACCESS

2005 Volume 69 Issue 11 Pages 2117-2121

DOI https://doi.org/10.1271/bbb.69.2117

Details

Abstract

To investigate the role of claudin-6 in adipogenesis, claudin-6 mRNA was examined in adipose tissues and adipocyte differentiation. Claudin-6 mRNA was found to be differentially expressed in four different adipose tissues, and up-regulated in each fat depot of mice fed a high-fat diet as compared to a normal-fat diet. Levels of claudin-6 transcripts were increased during differentiation of 3T3-L1 cells in vitro. Moreover, small interfering RNA (siRNA)-mediated reduction of claudin-6 mRNA inhibited differentiation of 3T3-L1 cells. These results suggest that claudin-6 is another important regulator in adipogenesis and fat deposition.

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