Purification and Characterization of Aldoxime Dehydratase of the Head Blight Fungus, Fusarium graminearum

Yasuo KATO; Yasuhisa ASANO

doi:10.1271/bbb.69.2254

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Purification and Characterization of Aldoxime Dehydratase of the Head Blight Fungus, Fusarium graminearum

Yasuo KATO, Yasuhisa ASANO

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Keywords: aldoxime dehydratase, aldoxime-nitrile pathway, nitrilase, Fusarium graminearum, characterization

JOURNAL FREE ACCESS

2005 Volume 69 Issue 11 Pages 2254-2257

DOI https://doi.org/10.1271/bbb.69.2254

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Abstract

Fungal aldoxime dehydratase (Oxd) of Fusarium graminearum MAFF305135 was purified and characterized for the first time from its overexpressing Escherichia coli transformant. The enzyme showed about 20% identity with known Oxds, and had similar enzymatic properties with nitrilase-linked Oxd from the Bacillus strain. It belongs to a group of phenylacetaldoxime dehydratases (EC 4.99.1.7), based on its substrate specificity and kinetic analysis.

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