High-Level Production of Astaxanthin by Xanthophyllomyces dendrorhous Mutant JH1 Using Statistical Experimental Designs

Abstract

Medium composition was optimized for high-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs. Glucose and yeast extract were the most important factors affecting astaxanthin production. Glucose 3.89%, yeast extract 0.29%, KH₂PO₄ 0.25%, MgSO₄ 0.05%, MnSO₄ 0.02%, and CaCl₂ 0.01% were optimum for high-level production of astaxanthin. Under optimized conditions, the maximum concentration of astaxanthin obtained after 7 d of cultivation was 36.06 mg/l. The concentration of astaxanthin predicted by a polynomial model was 36.16 mg/l.