Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Molecular Cloning of Streptomyces Genes Encoding Vanillate Demethylase
Motohiro NISHIMURADaisuke ISHIYAMAJulian DAVIES
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JOURNALS FREE ACCESS

2006 Volume 70 Issue 9 Pages 2316-2319

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Abstract

The vanillate demethylase genes from Streptomyces sp. NL15-2K were cloned and sequenced. The vanA and vanB gene homologs, which encode the terminal oxygenase subunit (VanA) and the ferredoxin-type reductase subunit (VanB) of the enzyme respectively, were found in the sequenced 7.5-kb DNA region. Expression of the vanAB genes in Streptomyces lividans 1326 resulted in in vivo demethylation of veratric acid to vanillic acid.

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© 2006 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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