Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA; Yuko MATSUBARA; Yoshinori TANAKA; Hirotaka TAKAHASHI; Tatsuya AKAGI; Motoaki SEKI; Kazuo SHINOZAKI; Yaeta ENDO; Tatsuya SAWASAKI

doi:10.1271/bbb.90026

Biochemistry & Molecular Biology Notes

Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis

Akira NOZAWA, Yuko MATSUBARA, Yoshinori TANAKA, Hirotaka TAKAHASHI, Tatsuya AKAGI, Motoaki SEKI, Kazuo SHINOZAKI, Yaeta ENDO, Tatsuya SAWASAKI

Author information

Akira NOZAWA
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Systems and Structural Biology Center, RIKEN
Yuko MATSUBARA
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Yoshinori TANAKA
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Hirotaka TAKAHASHI
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Tatsuya AKAGI
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Motoaki SEKI
Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center
Kazuo SHINOZAKI
Gene Discovery Research Team, Gene Discovery Research Group, RIKEN Plant Science Center
Yaeta ENDO
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Systems and Structural Biology Center, RIKEN
Tatsuya SAWASAKI
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
Systems and Structural Biology Center, RIKEN

Keywords: Arabidopsis, wheat cell-free protein production system, transcription factor

JOURNAL FREE ACCESS

2009 Volume 73 Issue 7 Pages 1661-1664

DOI https://doi.org/10.1271/bbb.90026

View "Advance Publication" version (July 7, 2009).

Details

Abstract

We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5′-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.

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